ShortLong-Seq Reads Bioinformatics
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RNAseq Analysis: Count Matrix

Oct 6, 20233 min read

We learned how to quality control, align sequences and generate DEG analysis using the cufflink tool. What if I want to create just a count matrix? In...

RNAseq Analysis: Count Matrix

Identifying Sequence Differences: Variant Calling

Sep 8, 20236 min read

Variant Calling is used when one wants to identify differences in genomic sequence such as SNP (single nucleotide polymorphism), insertions, deletions...

Sequence Mapping, Part. 2

Mar 6, 202311 min read

De Novo Transcriptome Analysis (No Reference Based) · In this article, I will walk you through how to conduct a de novo transcriptome analysis. If you...

Sequence Mapping, Part. 1: Reference Based Assembly

Jan 16, 20237 min read

Reference Based Transcriptome Analysis (Genome Mapping) · Preliminary Preparation: Before we start, there are a couple of tools (hyperlinked) to...

Sequence Mapping, Part. 1: Reference Based Assembly

High Throughput Sequence Data Pipeline, Part. 2: Sequence Mapping

Dec 30, 20222 min read

Reference vs. De novo Transcriptome Assembly · Continuing in our series, the next part I will be discussing is sequence mapping. Referring back to this...

High Throughput Sequence Data Pipeline, Part. 2: Sequence Mapping

Sequence Data Quality Control, Part. 2: Trimmomatic

Dec 19, 20223 min read

Using Trimmomatic to Clean High Throughput Sequence Data · Now that you have assessed the quality of your sequenced data using FastQC, it's time to...

Sequence Data Quality Control, Part. 2: Trimmomatic